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目的:比较贵州不同产地太子参中太子参环肽B的含量,为确立贵州太子参药材种植适宜性区域提供依据。方法:采用高效液相色谱法,以太子参环肽B的含量为考察依据。色谱条件:色谱柱为SUPELCO C18柱(5μm,4.6 mm×250 mm),以乙腈—水为流动相,流速为0.9 ml/min,检测波长203 nm,柱温为40℃,进样量:20μl。结果:在测定的15个采样点的样品只有3个采样点的样品中太子参环肽B的含量达到原药典的要求。
Objective: To compare the content of Rhizoma Panax ginseng Pepper B in different areas of Guizhou, and to provide the basis for establishing the appropriate area for the cultivation of Radix Pseudostellariae in Guizhou. Methods: The HPLC method was used to determine the content of EPG B in root. Chromatographic conditions: The column was SUPELCO C18 (5 μm, 4.6 mm × 250 mm). The mobile phase consisted of acetonitrile-water with a flow rate of 0.9 ml / min and a detection wavelength of 203 nm. The column temperature was 40 ℃. . Results: In the sample with 15 sampling points, the content of heteronuclear peptide B in the sample with only 3 sampling points reached the requirements of the original pharmacopoeia.