论文部分内容阅读
目的:观察滋肾调冲法代表方功血宁Ⅱ号方对骨髓内皮祖细胞(EPCs)促进黄体血管新生的影响,探讨该法健全黄体功能的作用机制。方法:建立大鼠骨髓移植假孕模型,随机分为移植对照组、功血宁Ⅱ号组和乌鸡白凤丸组,另取未骨髓移植大鼠为假孕对照组。在黄体早中晚期,各组处理相应数目动物,摘取卵巢。采用PCR法检测黄体中供体雄鼠来源的Sry基因的表达;原位杂交法检测Sry基因阳性细胞;原位杂交合免疫组化双标染色,观察EPCs向血管内皮细胞分化情况;免疫组化法检测黄体微血管密度(MVD)。结果:骨髓移植各组的黄体中均检测到Sry基因和双标记阳性细胞,假孕对照组未见;Sry基因的表达量均显著高于假孕对照组(P<0.01);功血宁Ⅱ号组显著升高(P<0.05)。黄体MVD各组早期较少,中期显著增多,晚期明显减少(P<0.01);功血宁Ⅱ号组黄体中期MVD显著增多(P<0.01)。结论:滋肾调冲法能够将骨髓EPCs归巢于黄体,并分化为血管内皮细胞,使黄体MVD增加,促进黄体的血管新生,从而达到健全黄体功能的作用。
OBJECTIVE: To observe the effect of Zishen Tiaochong recipe on behalf of progesterone-derived progenitor cells (EPCs) on the corpus luteum angiogenesis and to explore the mechanism of perfecting luteal function. Methods: The rat model of pregnancy induced by bone marrow transplantation was established and randomly divided into transplantation control group, gingyouning Ⅱ group and Wuji Baifengwan group, and the other non-bone marrow transplantation rats were control group. Early and late in the corpus luteum, each group treated the corresponding number of animals, ovaries were removed. PCR was used to detect the expression of Sry gene in donor corpus luteum; Sry gene positive cells were detected by in situ hybridization; double staining by immunohistochemistry in situ hybridization was used to observe the differentiation of EPCs into vascular endothelial cells; Law test luteal microvessel density (MVD). Results: The Sry gene and double positive cells were detected in the corpus luteum of each group. The expression of Sry gene was significantly higher than that of the pregnant control group (P <0.01) Number group was significantly higher (P <0.05). Luteal MVD in each group was lower in the early stage, significantly increased in the middle stage, and significantly decreased in the late stage (P <0.01). MVD in the middle of the corpus luteum was significantly increased (P <0.01). Conclusion: Nourishing kidney transfer method can bone marrow EPCs homing in the corpus luteum, and differentiate into vascular endothelial cells, the luteal MVD increased to promote corpus luteum angiogenesis, so as to achieve the role of sound corpus luteum function.