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生长素在植物发育中的各个阶段都起着重要作用,而生长素反应因子(auxin response factors,ARF)特异性的调节生长素反应基因的表达,是植物细胞中重要的一类转录因子家族。在拟南芥、玉米、水稻等模式作物中先后克隆了一些ARF基因,但是高粱作为一种重要的经济作物,这方面的研究未见报道。随着高粱全基因组序列的公布,利用基因组序列分析ARF基因的数目、结构、进化具有重要的意义。利用公布的高粱全基因组数据,利用DNATOOLS、BLAST、MEGA4.0以及Genomepixelizer等生物信息学软件对高粱(Sorghum bicolor)生长素反应因子ARF基因的数量、物理位置、系统进化树、氨基酸序列及保守基序(motif)的保守性等进行分析。结果表明,在高粱全基因组中共有26个ARF基因,26个ARF基因根据其进化关系分为A、B、C 3类;通过对全基因组内ARF基因进行物理位置和基因家族分析,发现高粱基因组中ARF基因存在明显的基因复制现象,基因的复制对高粱基因组中ARF基因数量扩张起到了重要的作用。
Auxin plays an important role in all stages of plant development. Auxin response factors (ARFs) regulate the expression of auxin response genes and are important families of transcription factors in plant cells. Some ARF genes have been cloned from Arabidopsis, maize and rice, but sorghum has been reported as an important cash crop. With the publication of the whole genome sequence of sorghum, it is of great significance to analyze the number, structure and evolution of ARF genes by using genomic sequences. Based on the published whole genome data of sorghum, the number, physical location, phylogenetic tree, amino acid sequence and conserved motif of ARF gene in sorghum bicolor were studied by using bioinformatics software such as DNATOOLS, BLAST, MEGA4.0 and Genomepixelizer. The conservation of motif and so on. The results showed that there are 26 ARF genes in the whole sorghum genome and 26 ARF genes are classified into A, B, and C classes according to their evolutionary relationships. By analyzing the genome-wide ARF gene location and gene family analysis, it was found that sorghum genome ARF gene in the presence of significant duplication of genes, gene duplication of sorghum genome ARF gene has played an important role in the expansion.