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以人宫颈癌为材料,同一份样品用四个实验指标来检测和分析癌蛋白P21的表达变化。结果发现:宫颈癌组织中存在有P21的表达;表达蛋白的分子量约为21KD;表达的主要部位在恶变细胞胞浆和胞膜中;表达水平高于慢性炎症约六倍。实验结果在临床应用中得到了验证。54例宫颈石蜡切片用ABC免疫酶标法进行了检测。宫颈癌组P21阳性率和阳性强度明显高于慢性炎症组,二者有显著性差异(P<0.05)。
Taking human cervical cancer as material, the same sample was used to detect and analyze the expression changes of oncoprotein P21 with four experimental indexes. The results showed that there was expression of P21 in cervical cancer tissues; the molecular weight of the expressed protein was about 21 kD; the expression was mainly located in the cytoplasm and membrane of malignant cells; the expression level was about six times higher than chronic inflammation. The experimental results have been validated in clinical application. 54 cases of cervical paraffin sections with ABC immunoenzyme method was tested. The positive rate and positive rate of P21 in cervical cancer group were significantly higher than those in chronic inflammation group (P <0.05).