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目的:建立多波长同时测定3种獐牙菜属植物中6种活性成分含量的高效液相色谱方法。方法:采用HypersilBDS色谱柱(4.6 mm×200 mm,5μm);流动相为乙腈-0.5%磷酸水溶液,梯度洗脱,流速为1.0 mL.min-1;检测波长为240,274,325,334 nm;柱温25℃。结果:在此色谱条件下,各组分在60 min内均得到较好分离;獐牙菜苦苷、龙胆苦苷、去甲当药苷、雏菊叶龙胆苷、去甲基雏菊叶龙胆酮和雏菊叶龙胆酮分别在0.520~20.8,0.202~8.06,0.107~4.28,0.097~3.86,0.094~3.77,0.101~4.02μg线性良好,相关系数r均为0.999 9,平均回收率分别为98.7%,98.1%,98.3%,98.8%,98.1%,98.6%,RSD均小于3.0%(n=6)。结论:本方法简单、快速、准确,重现性好,可为全面评价和控制獐牙菜属药材质量提供参考。
OBJECTIVE: To establish a high performance liquid chromatographic method for simultaneous determination of 6 active ingredients in three kinds of Swertia by multiwavelength. Methods: Hypersil BDS column (4.6 mm × 200 mm, 5 μm) was used. The mobile phase consisted of acetonitrile-0.5% phosphoric acid solution with a gradient of 1.0 mL · min-1. The detection wavelength was 240, 274, 325 and 334 nm. Results: Under these chromatographic conditions, all the components were well separated in 60 min. Swertia glycosides, gentiopicroside, nordin, daisy leaf gentianoside, demethyl daisy Cholesterol and daisy chondroitin had good linearity in the range of 0.520-20.8,0.202-8.06,0.107-4.28,0.097-3.86,0.094-3.77,0.101-4.02μg respectively, the correlation coefficient r was 0.999 9, the average recoveries were 98.7%, 98.1%, 98.3%, 98.8%, 98.1%, 98.6% respectively. The RSDs were less than 3.0% (n = 6). Conclusion: The method is simple, rapid, accurate and reproducible, which can provide a reference for the comprehensive evaluation and control of the quality of Swertia.