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目的了解中国ＴＴＶ基因分型状况。方法采用套式ＰＣＲ检测Ｄ８、 Ｄ１０、 Ｄ２２、 Ｄ２５、 Ｄ２９血清中ＴＴＶ ＤＮＡ，限制性内切酶和ＰＣＲ产物直接测序技术进行酶切和序列分析。结果 Ｎ２２～ Ｄ８存在 ＢｓｔＵＩ特异性切点， Ｇ１ｂ～Ｄ１０无 ＢｓｔＵＩ切点，有ＥｃｏＲＩ切点，Ｇ２ａ、Ｄ２５、Ｄ２９序列均存在Ｈａｅ和Ｔｓｐ５０９切点，Ｇ２ｂ－Ｄ２２除存在ＨａｅⅢ切点之外还具有ＭｂｏⅠ切点。Ｐａｔ３～ＴＴｈ６仅有Ｈａｅ Ⅲ切点。结论ＡＢ００８３９４～Ｄ８具有相同的切点可能均属ｌａ型，Ｇ１ｂ－Ｊｃａｓｅ５切点相同可能为１ｂ型，ＴＴｈ１～１和ＴＴｈ１～２可能属１型中的其它亚型。Ｄ２５～Ｄ２９酶切点相同为２ａ型，Ｇ２ｂ～Ｄ２２切点相同为２ｂ型，Ｐａｔ３～ＴＴｈ６切点相同可能为２型中的其它亚型。
Objective To understand the status of TTV genotyping in China. Methods TdDNA of D8, D10, D22, D25 and D29 were detected by nested PCR and restriction endonucleases and direct sequencing of PCR products were digested and sequenced. Results There were BstUI specific cleavage sites on N22 ~ D8, no BstUI cleavage sites on G1b ~ D10, EcoRI cleavage sites, Hae and Tsp509 cleavage sites on G2a, D25 and D29 sequences, G2b-D22 had HaeⅢ cleavage sites Mbo I cut point. Pat3 ~ TTh6 only Hae Ⅲ cut point. Conclusion The same point of cleavage between AB008394 and D8 may belong to la type. The same point of G1b-Jcase5 may be type 1b, and TTh1-1 and TTh1-2 may belong to other subtypes of type 1. D25 ~ D29 digestion point the same as 2a type, G2b ~ D22 same point of the same type 2b, Pat3 ~ TTh6 same point may be other subtypes of type 2.