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LEAFY基因在植物的成花转变中起重要作用。在拟南芥中,降低LEAFY基因的表达使开花延迟,并且增加分枝。利用RNAi技术降低大白菜LEAFY基因表达,获得晚抽薹开花的转基因大白菜。开花时转基因大白菜的株高明显低于对照,而叶片数、叶面积和侧枝数则高于对照;转基因植株的LEAFY基因表达明显降低,导致大白菜抽薹开花延迟。因此可以通过降低LEAFY基因表达来延迟需低温春化作用长日植物开花。也为利用生物技术获得晚抽薹转基因大白菜提供了理论依据。
The LEAFY gene plays an important role in transplanting flowers into plants. In Arabidopsis, reducing expression of the LEAFY gene delays flowering and increases branching. The RNAi technique was used to reduce the LEAFY gene expression in Chinese cabbage and obtain the genetically modified transgenic Chinese cabbage which was late bolting and flowering. At the flowering stage, the plant height of transgenic Chinese cabbage was significantly lower than that of the control, while the number of leaves, leaf area and collateral number were higher than those of the control. The LEAFY gene expression of transgenic plants was significantly reduced, leading to delayed flowering of Chinese cabbage. Therefore, it is possible to delay the flowering of long-day plants under the effect of low-temperature vernalization by reducing LEAFY gene expression. It also provided a theoretical basis for using biotechnology to obtain late bolting transgenic cabbage.