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目的:研究酪氨酸蛋白激酶抑制剂Genistein诱导人唾液腺腺样囊性癌细胞株SACC-83细胞周期阻滞的分子机制。方法:用Genistein处理体外培养的SACC-83细胞,采用Western印迹技术检测CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达,并利用电泳凝胶成像分析软件,对其结果进行量化分析,采用SPSS11.5统计软件对结果进行方差分析。结果:随着Genistein作用时间的延长和浓度的增加,CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达明显减少。SACC-83细胞经220μmol/LGenistein作用3d,其CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达量分别是对照组的58%、64%和46%、43%,差异非常显著(P<0.01)。结论:Genistein诱导SACC-83细胞周期阻滞于G2/M期,与其下调CyclinB1、Cdk1和CyclinD1、Cdk4蛋白的表达有关。
AIM: To investigate the molecular mechanism of tyrosine kinase inhibitor Genistein in inducing human salivary adenoid cystic carcinoma cell line SACC-83 cell cycle arrest. Methods: The SACC-83 cells cultured in vitro were treated with Genistein. The expression of CyclinB1, Cdk1, CyclinD1 and Cdk4 protein was detected by Western blotting. The results were analyzed by electrophoresis gel imaging software and analyzed by SPSS11.5 The software performs an analysis of variance on the results. Results: The expression of CyclinB1, Cdk1, CyclinD1 and Cdk4 decreased with the increase of Genistein time and concentration. The expression of CyclinB1, Cdk1, CyclinD1 and Cdk4 in SACC-83 cells treated with 220μmol / LGenistein for 3 days were 58%, 64%, 46% and 43% of the control group respectively. The difference was significant (P <0.01). Conclusion: Genistein can induce cell cycle arrest at G2 / M phase in SACC-83 cells, which is related to its downregulation of CyclinB1, Cdk1, CyclinD1 and Cdk4 protein expression.