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目的了解湘潭地区乙型肝炎病毒(HBV)基因型流行情况及其临床意义,并对HBV基因分型试剂盒进行评价。方法用HBV基因分型试剂对湘潭地区95例HBV感染者的样本进行检测,并对PCR产物进行测序分析。结果 HBV基因分型试剂盒检测结果与测序的符合率为100%,湘潭地区HBV各种基因型分布比例:B型占74.74%,C型占11.58%,B、C型混合感染占13.68%。无症状携带者组与慢性重型肝炎组.慢性乙型肝炎组与慢性重型肝炎组及肝硬化组之间HBV B型和C型感染率存在显著性差异,其中慢性重型肝炎组及肝硬化组C型感染率要明显高于其他组。结论 HBV基因分型检测试剂盒检测结果是准确、简便的,具有临床推广和应用价值。湘潭地区流行的HBV基因型主要为B型和C型,其中B型为优势基因型,具有其自身的特点。
Objective To understand the prevalence and clinical significance of hepatitis B virus (HBV) genotypes in Xiangtan area and to evaluate the HBV genotyping kit. Methods HBV genotyping reagents were used to detect 95 cases of HBV infection in Xiangtan area, and the PCR products were sequenced. Results The coincidence rate of HBV genotyping kit and sequencing was 100%. The distribution of HBV genotypes in Xiangtan region was 74.74% in type B, 11.58% in type C and 13.68% in type B and C. Asymptomatic carrier group and chronic severe hepatitis group.Chronic hepatitis B group and chronic severe hepatitis group and cirrhosis group HBV B and C infection rates were significantly different, including chronic severe hepatitis group and cirrhosis group C Type infection rate was significantly higher than other groups. Conclusion The detection results of HBV genotyping kit are accurate and simple, and have clinical application and promotion value. The prevalence of HBV genotypes in Xiangtan are mainly B type and C type, of which B type is the predominant genotype and has its own characteristics.