High-performance silicalite-1 membranes were synthesized on silica tubes by in-situ hydrothermal synthesis. By using the “solution-filling (SF)” method, the average flux of membranes with the SF method was improved by about 25% compared to that of the membranes without using the SF method; the flux and the separation factor of the membranes prepared with the SF method for an ethanol/water mixture at 60 ℃ were 0.99 kg/(m2·h) and 73, respectively. It was found that the membranes synthesized on silica tubes exhibited high thermal stability and high reproducibility, and the relatively standard deviations (R.S.D.) of the average flux and separation factor were only 9.6% and 5.6%, respectively, which suggests that the silica support is more suitable than other kinds of supports for preparing high-performance silicalite-1 membranes. High-performance silicalite-1 membranes were synthesized on silica tubes by in-situ hydrothermal synthesis. By using the “solution-filling (SF)” method, the average flux of membranes with the SF method was improved by about 25% to that of the membranes without using the SF method; the flux and the separation factor of the membranes prepared with the SF method for an ethanol / water mixture at 60 ° C were 0.99 kg / (m2 · h) and 73, respectively. It was found that the papers synthesized on silica tubes exhibited high thermal stability and high reproducibility, and the relatively standard deviations (RSD) of the average flux and separation factor were only 9.6% and 5.6% respectively, which suggests that the silica support is more suitable than other kinds of supports for preparing high-performance silicalite-1 membranes.