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[目的]探讨锰染毒条件下大鼠大脑热休克蛋白27(HSP27)和70(HSP70)的变化规律。[方法]健康2月龄雄性SD大鼠50只,随机分为5组:生理盐水对照组和Mn2+染毒2.5、5.0、10.0、20.0mg/kg组(分别以A、B、C、D和E组表示)。每只大鼠每天腹腔注射染毒0.5mL,连续30d,处死大鼠后采用等离子体原子发射光谱(ICP-AES)测定脑组织锰含量。通过实时荧光定量PCR(QT-PCR)检测大鼠亚慢性锰染毒后脑组织中HSP27和HSP70mRNA的相对表达水平。[结果]不同剂量组脑组织中的锰含量与对照组相比差异具有统计学意义(P<0.01)。与对照组相比,B、C、D、E组HSP27mRNA的相对表达量增加,分别为对照组的2.00、5.51、8.78和6.61倍;HSP70mRNA的相对表达量也增加,分别是对照组的1.68、10.27、22.88和2.20倍。[结论]锰可以调节大鼠脑组织HSP27和HSP70表达的变化。
[Objective] To investigate the changes of heat shock protein 27 (HSP27) and 70 (HSP70) in rat brain under manganese exposure. [Methods] Fifty healthy 2-month-old male Sprague-Dawley rats were randomly divided into 5 groups: normal saline control group and Mn2 + -treated groups of 2.5, 5.0, 10.0 and 20.0 mg / kg Group E said). Each rat was intraperitoneally injected with 0.5 mL per day for 30 days. The rats were sacrificed and the contents of manganese in the brain tissue were determined by ICP-AES. The relative expression levels of HSP27 and HSP70 mRNA in brain tissue were detected by real-time fluorescence quantitative PCR (QT-PCR). [Results] The contents of manganese in brain tissue of different dose groups were significantly different from those in control group (P <0.01). Compared with the control group, the relative expression levels of HSP27 mRNA in B, C, D and E groups were 2.00, 5.51, 8.78 and 6.61 times higher than those in the control group respectively; the relative expression levels of HSP70 mRNA also increased in control group (1.68, 10.27, 22.88 and 2.20 times. [Conclusion] Manganese can regulate the expression of HSP27 and HSP70 in rat brain.