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An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors (DNA template, Taq DNA polymerase, Mg~(2+), primer, and dNTP) and annealing temperature have been tested separately in this system. The results demonstrated the reaction efficiency was affected by these factors. Based on the results, a stable, productive and reproducible PCR system and cycling program for amplifying a ginseng SSR locus were obtained: 20 μL system containing 1.0 U Taq DNA polymerase, 2.0 mmol·L~(-1) Mg~(2+), 0.2 mmol·L~(-1) dNTPs, 0.3 μmol·L~(-1) SSR primer, 60 ng·μL~(-1) DNA template, performed with a program of 94°C for 5 min, 94°C for 30 s, annealing at 56.3°C for 30 s, 72°C for 1 min, 37 cycles, finishing at 72°C for 7 min, and storing at 4°C.
An orthogonal design was used to optimize SSR-PCR amplification system using Panax ginseng genomic DNA as template. Four levels of five factors (DNA template, Taq DNA polymerase, Mg 2+, primer, and dNTP) and annealing temperature have been based on the results, a stable, productive and reproducible PCR system and cycling program for amplifying a ginseng SSR locus were obtained: 20 μL system containing 1.0 U Taq DNA polymerase, 2.0 mmol·L -1 Mg 2+, 0.2 mmol·L -1 dNTPs, 0.3 μmol·L -1 SSR primer and 60 ng · μL -1 ) DNA template, performed with a program of 94 ° C for 5 min, 94 ° C for 30 s, annealing at 56.3 ° C for 30 s, 72 ° C for 1 min, 37 cycles, finishing at 72 ° C for 7 min , and storing at 4 ° C.