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Aim:To investigate the specific effects of Ca~(2+) on transgene expression duringelectroporation-mediated gene transfer in mice.Methods:Skeletal muscle andskin were subjected to in vivo electroporation with a luciferase reporter plasmid,with or without Ca~(2+) and various other ions.Results:For in vivo electroporation,the presence of just 10 mmol/L Ca~(2+) in the DNA solution drastically reduced theresulting transgene expression,to less than 5% of control values.Only Ca~(2+),notother ions,caused inhibition,and the effect was not tissue specific.Moresurprisingly,even when Ca~(2+) ions were delivered by electroporation before orafter DNA administration,similar effects were still observed.Conclusion:Theinhibitory effect of Ca~(2+) on in vivo gene transfer by electroporation is specific,ie,the inhibitory effect may be related to the cell membrane properties afterelectroporation and the subsequent resealing event.
Aim: To investigate the specific effects of Ca ~ (2+) on transgene expression duringelectroporation-mediated gene transfer in mice. Methods: Skeletal muscle and skin were subjected to in vivo electroporation with a luciferase reporter plasmid, with or without Ca ~ (2+ ) and various other ions. Results: For in vivo electroporation, the presence of just 10 mmol / L Ca ~ (2+) in the DNA solution drastically reduced the resulting transgene expression to less than 5% of control values. Only Ca ~ ( 2 +), not inhibited ions, caused inhibition, and the effect was not tissue specific. Moresurprisingly, even when Ca ~ (2+) ions were delivered by electroporation before orafter DNA administration, similar effects were still observed. Conlusion: The inhibition effect of Ca ~ (2+) on in vivo gene transfer by electroporation is specific, ie, the inhibitory effect may be related to the cell membrane properties afterelectroporation and the subsequent resealing event.