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目的 观察氯化锂协同温热对 Ｂ Ｇ Ｃ８２３ 人胃癌细胞的生物学效应。方法 通过克隆形成率、细胞生长曲线测定肿瘤细胞生长特性变化，用免疫组化染色测定细胞 Ｈｓｐ７０、ｐ５３ 表达变化。结果 研究表明氯化锂在一定范围内随着浓度的增加，对肿瘤细胞生长的抑制作用逐渐增强；４１℃温热对肿瘤细胞无显著抑制作用；两者合并作用时，克隆形成率进一步降低（ Ｐ＜ ０．０１），显示明显协同效应。免疫组化观察显示， Ｈｓｐ７０ 主要分布于细胞质，４１℃温热可诱导 Ｂ Ｇ Ｃ８２３ 细胞 Ｈｓｐ７０ 表达增加，但温热与氯化锂合并作用则使 Ｈｓｐ７０ 的表达显著抑制（ Ｐ＜ ０．０５）；ｐ５３主要分布于细胞核，氯化锂（１ ｍ ｇ／ｍ ｌ）及合并作用对ｐ５３ 表达有轻度抑制作用。结论 单价阳离子锂与温热合并有一定协同抑制肿瘤细胞生长作用。其中抑制 Ｈｓｐ７０ 表达，阻止热耐受的产生可能是其协同机理之一。
Objective To observe the biological effects of lithium chloride synergistic warming on BGC-823 human gastric cancer cells. Methods The growth characteristics of tumor cells were determined by cloning efficiency and cell growth curve. The expression of Hsp70 and p53 in cells was determined by immunohistochemical staining. The results showed that the inhibitory effect of lithium chloride on the growth of tumor cells gradually increased with the increase of the concentration within a certain range; 41°C warming had no significant inhibitory effect on the tumor cells; when the two combined effect, the cloning rate further decreased ( P < 0.01), showing a clear synergistic effect. Immunohistochemical observation showed that Hsp70 was mainly distributed in the cytoplasm and that 41°C warming could induce the increase of Hsp70 expression in BGC-823 cells, but the combination of warming and lithium chloride significantly inhibited the expression of Hsp70 (P<0.05). P53 mainly distributed in the nucleus, lithium chloride (1 m g/ml) and the combined effect of mild inhibition of p53 expression. Conclusion The monovalent cationic lithium combined with warming has a certain synergistic effect in inhibiting the growth of tumor cells. The inhibition of Hsp70 expression and the prevention of heat tolerance may be one of its synergistic mechanisms.