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Gross saponins of Tribulus terrestris(GSTT) have exact effect on cardiovascular and cerebrovascular diseases.But as a mixture,the specific efficient component of GSTT is still unknown.Nine monomers of spirostanol saponins were isolated and idendified as JA―JI(named transitorily) by means of NMR spectrometry.After bio-activity screening on them,we defined that monomers tigogenin 3-O-β-D-xylopyranosyl(1→2)-[β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-galactopyranoside(compound JB) and hecogenin3-O-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(compound JG) have cytoprotective bio-activity.Compound JB display effective dose in 10-8 and 10-9 mol/L,and JG in 10-6―10-9 mol/L.Survival rate,lactate dehydrogenase(LDH) and apoptosis also show that JB(at dose 10-8,10-9 and 10-10 mol/L) can protect myocardial injury caused by hypoxia/reoxygenation(H/R).While morphology change also shows JG has cytoprotective bio-activity.
Gross saponins of Tribulus terrestris (GSTT) have exact effect on cardiovascular and cerebrovascular diseases. But as a mixture, the specific efficient component of GSTT is still unknown. Nine monomers of spirostanol saponins were isolated and idendified as JA-JI (named transitorily) by means of NMR spectrometry. After bio-activity screening on them, we define that monomers tigogenin 3-O-β-D-xylopyranosyl (1 → 2) - [β- D- xylopyranosyl (1 → 3) glucopyranosyl (1 → 4) - [α-L-rhamnopyranosyl (1 → 2)] - β-D-galactopyranoside (compound JB) and hecogenin 3-O- β-D-glucopyranosyl Compound JG has cytoprotective bio-activity. Comp JB efficacy dose in 10-8 and 10-9 mol / L, and JG in 10-6-10-9 mol / L.Survival rate, lactate dehydrogenase (LDH) and apoptosis also show that JB (at doses 10-8, 10-9 and 10-10 mol / L) can protect from injury caused by hypoxia / reoxygenation (H / R) .While morphology change also shows JG has cytoprotective bio-activity.