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目的:探讨低糖高乳酸环境对宫颈癌细胞生存的影响,以及对EGFR-m TOR通路的调节作用。方法:将Hela细胞培养于常糖(葡萄糖10mmol/L)、低糖(葡萄糖3mmol/L)、高乳酸(葡萄糖10mmol/L,乳酸2.5mmol/L)、低糖高乳酸(葡萄糖3mmol/L,乳酸2.5mmol/L)4种环境下,CCK-8法测定Hela细胞的生长抑制率,流式细胞术测定细胞周期。荧光实时定量PCR法检测EGFR和m TOR mRNA水平表达。结果:与常糖组相比,高乳酸组的细胞抑制率显著升高(P<0.01),48h细胞G1/G0期比例显著升高(P<0.01),细胞凋亡率、EGFR和m TOR mRNA表达水平均无变化。与常糖组相比,低糖组的细胞抑制率显著升高(P<0.01),48h细胞凋亡率显著降低(P<0.01),细胞各周期比例变化与常糖组无差异,EGFR表达水平降低(P<0.05)。与常糖组相比,低糖高乳酸组的细胞抑制率显著升高(P<0.01),但低于低糖组(P<0.01)和高乳酸组(P<0.05),48h G1/G0期比例显著升高(P<0.01),细胞诱导凋亡率显著升高(P<0.01),EGFR和m TOR mRNA表达水平均显著升高(P<0.01)。结论:Hela细胞在低糖高乳酸环境中的存活状况好于单纯低糖和单纯高乳酸环境,且伴随着EGFR和m TOR基因表达水平上升。
Objective: To investigate the effect of low-sugar, high-lactate environment on the survival of cervical cancer cells and the regulation of EGFR-m TOR pathway. Methods: Hela cells were cultured in the medium of glucose (10 mmol / L glucose, glucose 3 mmol / L), lactate (glucose 10 mmol / L, lactate 2.5 mmol / L) mmol / L), the inhibition rate of Hela cells was determined by CCK-8 method and the cell cycle was determined by flow cytometry. Real-time quantitative PCR was used to detect the expression of EGFR and m TOR mRNA. Results: Compared with normal sugar group, the cell inhibition rate of high lactate group was significantly increased (P <0.01), the ratio of G1 / G0 phase was significantly increased (P <0.01), the apoptosis rate, EGFR and m TOR No change in mRNA expression level. Compared with the normal sugar group, the cell inhibition rate of low glucose group was significantly increased (P <0.01), the apoptosis rate of 48h was significantly decreased (P <0.01), the proportion of cells in each cycle was no difference with the normal sugar group, EGFR expression level Decreased (P <0.05). Compared with the normal sugar group, the cell inhibition rate in the low glucose and high lactate groups was significantly increased (P <0.01), but lower than that in the low glucose group (P <0.01) and the high lactate group (P <0.05) (P <0.01). The apoptosis rate of cells was significantly increased (P <0.01), and the expression of EGFR and mTOR mRNA was significantly increased (P <0.01). CONCLUSION: The survival of Hela cells in hypoglycemic and lactic acid is better than those in simple hypoglycemic and simple hyperlactic acid, and the expression of EGFR and m TOR are up-regulated.