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以‘妃子笑’荔枝(Litchi chinenesis)为试材,通过PCR方法克隆了荔枝类甜蛋白基因Lc TLP(登录号JF682821)的c DNA全长和完整开放阅读框(ORF)对应的g DNA序列。序列分析表明:该基因无内含子,c DNA序列含有一个672 bp的ORF,编码223个氨基酸残基序列。荧光定量PCR结果表明:Lc TLP在花中表达量最高,其次是果皮,在果肉中表达量最低;在果实发育过程中,果皮中Lc TLP表达量先上升后下降,采后果皮中的表达量高于采前,炭疽菌侵染诱导Lc TLP的表达,失水和低温能抑制Lc TLP的表达。
Using the Litchi chinenesis as test material, the full length cDNA of Litchi thaumatin Lc TLP (accession number JF682821) and the g DNA sequence corresponding to the complete open reading frame (ORF) were cloned by PCR. Sequence analysis showed that there was no intron in the gene, and the c DNA sequence contained a 672 bp ORF encoding a 223 amino acid residue sequence. The result of real-time PCR showed that Lc TLP had the highest expression level in flower, followed by peel, and lowest in pulp. During fruit development, the expression of Lc TLP in peel increased first and then decreased, Higher than the pre-mining, anthrax infection induced Lc TLP expression, dehydration and hypothermia can inhibit Lc TLP expression.