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目的:探讨ADAM23基因mRNA及蛋白在结直肠癌中的表达规律及其临床意义.方法:我院2007-03/2008-03手术治疗的结直肠癌患者45例,分别取结直肠癌组织标本以及配对癌旁组织标本,应用逆转录-聚合酶链反应(RT-PCR)和免疫组织化学方法分析患者ADAM23基因mRNA及蛋白的表达进行.结果:结直肠癌组织中ADAM23基因mRNA及蛋白的表达明显降低(37.8%vs95.6%,28.9%vs86.7%,均P<0.01),且其表达与患者的年龄、性别以及肿瘤的大小无关,而与结直肠癌的分化程度、浸润深度、淋巴结转移和临床分期关系密切(χ2=5.688,14.79,11.8172,11.8172,P<0.01或0.05).结论:ADAM23参与了结直肠癌的发展过程,并与结直肠癌的部分生物学行为有关,可能成为结直肠癌诊断和判断其生物学特性的重要的分子生物学检测指标.
Objective: To investigate the expression of ADAM23 mRNA and protein in colorectal cancer and its clinical significance.Methods: Forty-five patients with colorectal cancer underwent surgery from March 2007 to March 2008 in our hospital, The paired para-cancerous tissue samples were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical methods for the expression of ADAM23 gene mRNA and protein.Results: The expression of ADAM23 mRNA and protein in colorectal cancer tissues was significantly (37.8% vs95.6%, 28.9% vs86.7%, all P <0.01), and the expression was not related to the patient’s age, sex and the size of the tumor, but correlated with the degree of differentiation, depth of invasion, lymph node Metastasis and clinical stage (χ2 = 5.688,14.79,11.8172,11.8172, P <0.01 or 0.05) .Conclusion: ADAM23 is involved in the development of colorectal cancer and may be part of the biological behavior of colorectal cancer, Rectal cancer diagnosis and judgment of its biological characteristics of important molecular biological test indicators.