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为了研究阿特拉津和毒死蜱单一及联合暴露对东北小鲵(Hynobius leechii)蝌蚪抗氧化酶活性的影响,实验选择不同浓度、不同时间点阿特拉津和毒死蜱单一及联合暴露对东北小鲵蝌蚪超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)及丙二醛(MDA)的影响。结果表明,随着暴露时间的增加及各处理组浓度的升高,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性明显降低,丙二醛(MDA)水平明显上升,从而说明阿特拉津和毒死蜱单一及联合暴露会对东北小鲵蝌蚪的抗氧化酶系活性产生影响,进而产生毒理效应。当暴露21 d后,通过恢复实验,发现恢复末期与暴露末期相比,低浓度组和中浓度组上述3种酶活性变化不显著,而高浓度组上述3酶活性均有明显改善。
In order to study the effects of single and combined exposure of atrazine and chlorpyrifos on the antioxidant enzyme activity of Tadpoles of Hynobius leechii, we selected single and combined exposure of atrazine and chlorpyrifos at different concentrations and at different time points, The effects of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) The results showed that the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) were significantly increased with the increase of exposure time and the concentration of each treatment group (MDA) and the level of malondialdehyde (MDA) increased significantly, which indicates that the single and combined exposure of atrazine and chlorpyrifos will affect the antioxidase activity of Tadpoles of Tadazipus fargesii and then produce toxicological effects. After 21 days of exposure, we found that there was no significant change in the activity of the above three enzymes in the low concentration group and the medium concentration group at the end of recovery compared with the end of the recovery, while the recovery of the above three enzymes in the high concentration group was significantly improved.