论文部分内容阅读
目的:观察益肾胶囊对糖尿病肾病(DN)大鼠模型肾组织Toll样受体4(toll-like receptor4,TLR4)、白细胞介素-6(IL-6)及白细胞介素-8(IL-8)表达水平的影响。方法:36只Wistar雄性大鼠随机分成正常对照组、DN模型组、益肾胶囊治疗组,每组12只,利用单侧肾切除加腹腔注射链脲佐菌素建立DN大鼠模型,连续给药12周。记录大鼠体重、观察24h尿蛋白定量、血肌酐(Scr)、尿素氮(BUN)变化;取肾组织行病理组织学观察;用免疫荧光方法检测肾组织中TLR4、IL-6及IL-8的表达水平的变化。结果:12周末,DN模型组大鼠的24h尿蛋白定量、Scr、BUN均高于正常对照组(P<0.05),肾组织中TLR4、IL-6及IL-8表达水平明显高于正常对照组(P<0.05);益肾胶囊治疗组大鼠24h尿蛋白定量、Scr、BUN均低于模型组(P<0.05),肾组织TLR4、IL-6及IL-8表达水平明显低于DN模型组(P<0.05)。结论:益肾胶囊可能通过调节肾组织TLR4、IL-6及IL-8的表达,减少DN的炎症反应,从而延缓了DN的进展。
Objective: To observe the effects of Yishen Capsule on toll-like receptor 4 (TLR4), interleukin-6 (IL-6) and interleukin-8 (IL- 8) the effect of expression level. Methods: Thirty-six Wistar male rats were randomly divided into normal control group, DN model group and Yishen Capsule treatment group, with 12 rats in each group. DN rats were established by unilateral nephrectomy and intraperitoneal injection of streptozotocin, Drug for 12 weeks. The weight of rats was recorded and the proteinuria, serum creatinine (Scr) and blood urea nitrogen (BUN) were observed 24hours. The pathological changes of kidney were observed. The expressions of TLR4, IL-6 and IL-8 Changes in the level of expression. Results: At the end of the 12th week, the urinary protein excretion, Scr and BUN in DN model group were significantly higher than those in normal control group (P <0.05). The expression of TLR4, IL-6 and IL-8 in DN group were significantly higher than those in normal control (P <0.05). The urinary protein excretion, Scr and BUN in YSJ group were lower than those in model group (P <0.05), and the expression of TLR4, IL-6 and IL-8 in kidney tissue were significantly lower than those in DN group Model group (P <0.05). Conclusion: Yishen Capsule may delay the progression of DN by regulating the expression of TLR4, IL-6 and IL-8 in kidney tissue and decreasing the inflammatory reaction of DN.