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目的:对从食源性致病菌考核样中检出的与致病性大肠埃希菌O114:K90(B)有交叉凝集反应的阪崎肠杆菌进行鉴定和分析。方法:参照GB/T4789《食品卫生微生物学检验》,对考核样品分离到的菌株采用血清学和生化反应进行检验。结果:从考核样中初步检出血清学凝集结果疑为O114:K90(B)致病性大肠埃希菌,经全面生化反应,微生物鉴定系统,鉴定为阪崎肠杆菌。讨论:在肠道致病菌的鉴定中,即便血清学反应符合的菌株也要结合全面生化反应结果做出最终的判断。
OBJECTIVE: To identify and analyze Enterobacter sakazakii which cross-agglutinated with pathogenic Escherichia coli O114: K90 (B), which was detected in foodborne pathogens. Methods: According to GB / T4789 “Microbiological examination of food hygiene”, the strains isolated from the test samples were tested by serological and biochemical reactions. Results: Serum agglutination was initially detected as O114: K90 (B) pathogenic Escherichia coli from the test sample, and was identified as Enterobacter sakazakii by comprehensive biochemical reaction and microbial identification system. Discussion: In the identification of enteric pathogens, even if the serological response of strains should be combined with comprehensive biochemical reaction results to make the final judgment.