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采用离体穗培养技术研究了蔗糖、谷氨酰胺和不同植物激素对杂交稻组合汕优 10号、KA 2 46 9穗培 4～ 6d籽粒的溶液、蔗糖吸收 ,以及籽粒淀粉积累和淀粉合成的酶活性的影响。在 5～ 15g L蔗糖浓度范围内 ,随着培养液中蔗糖浓度的提高 ,籽粒吸收溶液、蔗糖的量及淀粉积累、蔗糖合成酶、ADPG焦磷酸化酶、淀粉合成酶、淀粉分枝酶活性增加。降低培养液中谷氨酰胺浓度 ,籽粒的物质积累和籽粒活性下降。ABA对淀粉合成的酶有促进作用 ,其余 3种植物激素对离体穗培籽粒吸收溶液和蔗糖及淀粉合成的酶活性有负效应。本研究认为 ,水稻发育胚乳中淀粉的积累主要受蔗糖供应强度的影响
The effects of sucrose, glutamine and different plant hormones on in vitro panicle culture of Suyou 10, KA 2 46 9 spikelet cultivars from 4 to 6 days were studied in terms of in vitro panicle culture, sucrose absorption, starch accumulation and starch synthesis Effect of enzyme activity. Under the sucrose concentration range of 5 ~ 15g L, with the increase of sucrose concentration in the culture solution, the amount of grain absorption solution, sucrose content and starch accumulation, sucrose synthase, ADPG pyrophosphorylase, starch synthase, starch branching enzyme activity increase. Reduce the concentration of glutamine in the culture medium, the material accumulation of grain and the decrease of grain activity. ABA could promote starch synthesis, and the other three plant hormones had negative effect on the enzyme activity of isolated spike culture and sucrose and starch synthesis. This study suggests that starch accumulation in rice endosperm is mainly affected by the supply intensity of sucrose