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目的 :探讨银杏叶提取物对实验性大鼠肝纤维化的预防及治疗作用机制。方法 :采用四氯化碳腹腔注射诱导的大鼠肝纤维化模型 ,60只Wistar大鼠分为 5组 :模型组、银杏叶干预组、银杏叶治疗组、银杏叶组 ,另设正常对照组 ,应用HE染色观察大鼠肝组织的改变及Von Gieson胶原纤维特殊染色观察肝纤维化程度 ,生化法检测肝功能丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)、白蛋白 (ALB)水平 ,硫代巴比妥酸 (TBA)法检测肝组织丙二醛 (MDA)的含量 ,免疫组织化学染色法检测肝组织I型胶原、转化生长因子 β1(TGF β1 )的表达。结果 :银杏叶干预及治疗组与模型组相比 ,肝组织结构明显改善 ,纤维化增生程度减轻 ,肝功能改善 ,肝组织内MDA的含量以及I型胶原、TGF β1 的表达均明显低于模型组 (P <0 .0 1 )。结论 :银杏叶提取物可通过其抗氧化作用抑制肝星状细胞激活和转化 ,降低I型胶原、TGF β1 的表达 ,从而抑制和逆转肝纤维化形成
Objective: To explore the preventive and therapeutic mechanism of Ginkgo biloba extract on experimental liver fibrosis in rats. METHODS: Rat liver fibrosis model induced by intraperitoneal injection of carbon tetrachloride was used. Sixty Wistar rats were divided into five groups: model group, ginkgo leaf intervention group, ginkgo leaf treatment group, Ginkgo biloba leaf group, and normal control group. HE staining was used to observe the changes of liver tissue in rats and the degree of hepatic fibrosis was observed by special staining of Von Gieson collagen fibers. The liver function was measured by alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Albumin (ALB) levels, thiobarbituric acid (TBA) assay for malondialdehyde (MDA) content in liver tissue, and immunohistochemistry for detection of type I collagen and transforming growth factor β1 (TGFβ1) in liver tissue expression. RESULTS: Compared with the model group, the Ginkgo biloba leaf intervention group and the treatment group had significantly improved liver structure, reduced fibroplasia, improved liver function, liver MDA content, and type I collagen and TGF-beta1 expression were significantly lower than the model. Group (P <0. 0 1). Conclusion : Ginkgo biloba extract can inhibit the activation and transformation of hepatic stellate cells through its antioxidation and reduce the expression of type I collagen and TGF β1 , thus inhibiting and reversing liver fibrosis formation.