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目的检测胎儿生长受限(FGR)患者胎盘血管内皮生长因子(VEGFA)及其受体(VEGFR2)的表达,终末绒毛微血管超微结构的改变。方法通过Realtime-PCR、Western-blot和免疫组化,检测FGR胎盘VEGFA和VEGFR2的表达;通过透视电镜分析FGR胎盘终末绒毛微血管的结构。结果 FGR组VEGFA和VEGFR2的mRNA水平(1.28±0.48)、(1.73±0.29)和蛋白水平(0.71±0.32)、(0.61±0.25)明显低于正常妊娠组(4.21±1.72)、(3.71±0.89)(P<0.01);1.90±0.76、1.75±0.48(P<0.01);免疫组化分析FGR组VEFGA和VEGFR2均为低表达;FGR胎盘终末绒毛微血管呈退行性变。结论 FGR患者胎盘VEFGA和VEGFR2水平显著下降,可能是导致FGR发病机制的一个重要因素。
Objective To investigate the expression of vascular endothelial growth factor (VEGFA) and its receptor (VEGFR2) in placenta and the ultrastructure of terminal villi in patients with fetal growth restriction (FGR). Methods Real-time PCR, Western-blot and immunohistochemistry were used to detect the expression of VEGFA and VEGFR2 in FGR placenta. The structure of FGR placental terminal villi capillaries was analyzed by transmission electron microscopy. Results The mRNA and protein levels of VEGFA and VEGFR2 in FGR group were significantly lower than those in normal pregnancy group (1.28 ± 0.48, 1.73 ± 0.29, 0.71 ± 0.32, 0.61 ± 0.25, 3.71 ± 0.89, ) (P <0.01); 1.90 ± 0.76,1.75 ± 0.48 (P <0.01). The expression of VEFGA and VEGFR2 in FGR group was lower than that in FGR group. Conclusion The levels of VEFGA and VEGFR2 in placenta of patients with FGR are significantly decreased, which may be an important factor leading to the pathogenesis of FGR.