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目的:探讨广西巴马地区长寿群体与5-羟色胺转运体启动子区基因多态性(5-HTTLPR/rs25531)的关系。方法:选取379例广西巴马地区90岁以上壮族长寿老人作为长寿组和284例同地区60岁以上普通健康壮族老人作为对照组,采用PCRRFLP技术检测多态性,分析基因型在长寿中的作用。结果:①长寿组5-HTTLPR基因型频率分别为SS 55.67%、SL34.83%、LL 9.50%,等位基因S和L频率分别为73.09%和26.91%(LA=20.45%,LG=6.46%),与对照组比较,差异均有统计学意义(P<0.01)。3个等位基因(S,LA,LG)的频率分布与对照组比较差异明显(P=0.001)。②单因素Logistic回归分析显示LL分别和SS、SL存在统计学联系(P=0.001,OR=2.226,95%CI 1.374~3.608;P=0.039,OR=1.695,95%CI1.028~2.796)。结论:5-HTTLPR多态在广西巴马地区的分布,可能是该流域长寿的遗传学基础之一。
Objective: To investigate the relationship between the polymorphism of serotonin transporter (5-HTTLPR / rs25531) and longevity population in Bama region, Guangxi. Methods: A total of 379 Zhuang longevity elderly over 90 years old in Bama region of Guangxi were selected as the longevity group and 284 elderly healthy people over the age of 60 in the same region as control group. The polymorphisms were detected by PCR-RFLP to analyze the role of genotypes in longevity . Results: The frequencies of 5-HTTLPR genotype in longevity group were SS 55.67%, SL34.83%, LL 9.50%, allele S and L frequencies were 73.09% and 26.91% (LA = 20.45%, LG = 6.46% ), Compared with the control group, the difference was statistically significant (P <0.01). The frequency distribution of three alleles (S, LA, LG) was significantly different from that of the control group (P = 0.001). (2) Logistic regression analysis showed that there was statistical relationship between LL and SS and SL respectively (P = 0.001, OR = 2.226, 95% CI 1.374-3.608; P = 0.039, OR = 1.695,95% CI1.028-2.756). Conclusion: The distribution of 5-HTTLPR polymorphism in Bama region of Guangxi may be one of the genetic bases of longevity in this basin.