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豚鼠暴露于152加农炮发射—发(178dB,SPL)后24、72小时、1、2周和1月,用ABC法结合葡萄糖氧化酶-DAB-硫酸镍铵(ABC-GDN)行耳蜗CGRP免疫组化染色,反应产物用图象分析仪行定量测定,并记录震后不同时间ABR阈值,以探讨爆震后中耳损伤及耳蜗内、外毛细胞区CGRP-IR的变化与听阈的关系。结果提示爆震后中耳有损伤者阈移有减小的倾向。外毛细胞区含CGRP神经较内毛细胞区更易受损。橄榄耳蜗束(OCB)中CGRP对听功能有一定的保护作用。CGRP的减少可能降低了耳蜗对听损伤的耐受性。
Cochlear cochlear CGRP immunohistochemistry was performed by ABC method combined with glucose oxidase-DAB-nickel ammonium sulfate (ABC-GDN) at 24, 72 hours, 1, 2 weeks and 1 month after guinea pig exposure to 152 cannon- The chemiluminescence staining and the reaction products were quantitatively determined by image analyzer. The ABR thresholds were recorded at different times after shock to investigate the relationship between hearing loss threshold and CGRP-IR changes in the middle ear and cochlear inner and outer hair cells after detonation. The results suggest that there is a tendency for the threshold shifts to decrease after detonation of the middle ear. CGRP-containing nerves in outer hair cells are more vulnerable than inner hair cells. CGRP in olfactory cochlear bundle (OCB) has a protective effect on hearing function. A decrease in CGRP may reduce the tolerance of the cochlea to hearing impairments.