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为减少紫外线对黑附球菌Epicoccum purpurascens分生孢子的影响,提高分生孢子在田间的生物活性,在室内检测了6种紫外线吸收剂和4种光稳定剂之间的不同组合(复合型抗紫外线保护剂)对黑附球菌XF1菌株分生孢子抗紫外线的保护作用。结果表明:不同复合型抗紫外线保护剂对XF1分生孢子的保护效率差异较大,其中苯并三唑类与六甲基磷酰三胺的组合(a3b1)及苯甲酮与六甲基磷酰三胺的组合(a4b1和a5b1)保护作用较强,在253.7 nm波长紫外灯下照射40 min,保护效率均达90%以上;水杨酸苯酯类与六甲基磷酰三胺的组合(a1b1)对分生孢子的保护效率较低,仅为37.12%;水杨酸苯酯类、苯并三唑类、苯甲酮类与光稳定剂770(b2)的组合(a1b2、a3b2、a4b2和a5b2)则无保护作用。对于选出的3种复合型抗紫外线保护剂(a3b1、a4b1、a5b1),其最适宜的溶剂组合为V(正己烷)∶V(花生油)=1∶1,a4b1和a5b1使用时的质量浓度应大于7.5 mg/mL,而a3b1的质量浓度应大于10 mg/mL。采用波长为253.7 nm的紫外灯照射90 min,所选出的3种复合型抗紫外线保护剂对分生孢子的保护效率均保持在88%以上;照射180 min后,保护效率均大于63%。
In order to reduce the influence of ultraviolet light on the conidia of Epicoccum purpurascens and enhance the bioactivity of conidia in the field, different combinations of 6 kinds of UV absorbers and 4 kinds of light stabilizers were tested indoors Protective agent) against conidia of Candida albicans XF1 strain UV protection. The results showed that the protective efficacy of different composite UV protectants on conidia of XF1 varied greatly. Among them, the combination of benzotriazole and hexamethylphosphoric triamide (a3b1) and the combination of benzophenone and hexamethylphosphor The combination of acyl triamines (a4b1 and a5b1) showed stronger protective effect, and the protective efficiency reached more than 90% under the irradiation of 253.7 nm UV lamp for 40 min. The combination of phenyl salicylate and hexamethylphosphoric triamide (a1b1) had a low protective effect on conidia of only 37.12%; the combination of phenyl salicylates, benzotriazoles, benzophenones and light stabilizer 770 (b2) (a1b2, a3b2, a4b2 and a5b2) have no protective effect. The most suitable solvent combinations for the three composite UV protection agents (a3b1, a4b1 and a5b1) were V (n-hexane): V (peanut oil) = 1: 1, mass concentrations of a4b1 and a5b1 Should be greater than 7.5 mg / mL, and a3b1 mass concentration should be greater than 10 mg / mL. The protective efficacy of the three composite UV protectants on conidia maintained above 88% with irradiation of 253.7 nm UV lamp for 90 min. The protection efficiency was over 63% after 180 min exposure.