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在 pH5.8二甲酚橙与锌形成1:1的紫红色络合物,在570nm 有一最大吸收峰。以氢氧化钠分解试样,除去大部分干扰,少量的铜、铁、以硫脲及抗坏血酸掩蔽,大量的铝,以氟化铵掩蔽,用二甲酚橙显色后,进行分光光度测定锌,检出量0.24微克/毫升。试验部分一试剂和仪器所用试剂的纯度最好均为优级纯,以免杂质干扰.1 氢氧化钠:20%,水溶液盛于塑料瓶中(一级)。2 抗坏血酸:10%,当天配制(二级)。3 氟化铵:10%,盛于塑料瓶中(二级).4 二甲酚橙:0.1%,水溶液(二级)。5 乙酸钠-乙酸缓冲溶液:(pH5.8)24克乙酸钠,加1毫升冰乙酸,稀至100毫升;或者17克无水乙酸钠,加1毫升冰乙酸,用水稀至100毫升.在 pH 计
At pH 5.8 xylenol orange forms a 1: 1 fuchsine complex with zinc and has a maximum absorption at 570 nm. Sodium hydroxide decomposition sample, remove most of the interference, a small amount of copper, iron, with thiourea and ascorbic acid masking, a large number of aluminum, with ammonium fluoride masking, with xylenol orange color, the spectrophotometric determination of zinc , The detection of 0.24 micrograms / ml. Reagents and instruments used in the test section of the best purity of the reagents are excellent pure, so as to avoid impurities .1 sodium hydroxide: 20%, Sheng Sheng plastic bottles (a). 2 ascorbic acid: 10%, the day of preparation (two). 3 Ammonium fluoride: 10% in a plastic bottle (second grade) .4 Xylenol orange: 0.1%, aqueous solution (second grade). 5 Sodium acetate-acetate buffer solution: (pH5.8) 24 g sodium acetate, add 1 ml glacial acetic acid, dilute to 100 ml; or 17 g anhydrous sodium acetate, add 1 ml glacial acetic acid and dilute to 100 ml with water at pH meter