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目的:连续观察45 d给大鼠柴胡总皂苷醇洗脱精制样品导致大鼠长期毒性损伤程度及恢复情况。方法:分别给大鼠灌胃高、中、低剂量的柴胡总皂苷醇洗脱精制样品连续45 d,按柴胡总皂苷计算,高、中、低剂量组分别为60,30,15 mg·kg-1,除观察一般状况外,检测血常规,血清肝功、肾功、脂质代谢、糖代谢相关指标;剖杀大鼠,精密称取心、肝、脾、肺、肾脏质量,计算脏体比值;进行肝脏病理组织学检查。剩余大鼠停止给药,恢复30 d,观察大鼠恢复情况,并进行上述相同指标的检测。结果:不同剂量的柴胡总皂苷醇洗脱精制样品在给药后45 d可导致大鼠体重下降,导致血中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBI)、总蛋白(TPC)、尿素氮(BUN)水平明显增高,白蛋白(ALB)明显降低,导致肝脏质量和肝体比值增大,病理学检查可见不同程度的肝组织损伤;上述变化随剂量的增加而逐渐加重,与空白组比较有明显差异。对碱性磷酸酶(AKP)、血糖(Glu)、胆固醇(CHO)、肌酐(Cr)等指标和血常规影响不明显。恢复期大鼠上述各指标与空白组比较均无显著性差异。结论:长时间给予一定剂量的柴胡总皂苷醇洗脱精制品可造成对大鼠明显的蓄积毒性,其毒性损伤部位以肝、肾损伤为主,经过30 d恢复期观察,其脏器损伤基本可恢复。
OBJECTIVE: To observe the long-term toxicity and recovery of rat after intravenous administration of refined extract of Bupleurum saponin 45 days continuously. Methods: Rats were given high, medium and low doses of emodin saponin elution refined samples for 45 consecutive days, the saikosaponin calculated high, medium and low dose groups were 60,30,15 mg · Kg-1, in addition to observe the general situation, the detection of blood, serum liver function, kidney function, lipid metabolism, glucose metabolism related indicators; killed rats, accurately weighed heart, liver, spleen, lung, Calculate the dirty body ratio; liver histopathology. The remaining rats were stopped and recovered for 30 days. The recovery of rats was observed and the same indexes as above were tested. Results: Different doses of total saponins from Bupleurum officinalis extract could cause weight loss in rats 45 d after administration, leading to changes of alanine aminotransferase (ALT), aspartate aminotransferase (AST) , Total bilirubin (TBI), total protein (TPC), and blood urea nitrogen (BUN) were significantly increased and albumin (ALB) was significantly lower, resulting in an increase in liver mass and liver mass ratio. Pathological examination revealed varying degrees of liver Tissue damage; The above changes gradually increased with the increase of dose, compared with the blank group there are significant differences. The effects of AKP, Glu, CHO and creatinine were not obvious. There was no significant difference between the above indexes in recovery rats and the blank group. Conclusion: The long-term administration of a certain dose of Bupleurum saponin eluted refined products can cause significant accumulation of toxicity in rats, the toxic injury sites mainly liver and kidney damage, after 30 d recovery observed organ damage Basic recovery.