目的：探讨广州地区丙型肝炎病毒（ＨＣＶ）基因型分布的特点以及ＨＣＶ基因型与临床病情的关系。方法：病例选自广州居民，基因分型使用Ｏｋａｍｏｔｏ的“型特异性引物ＰＣＲ法”，并加以改进，基因型的命名采用国际上统一的Ｓｉｍｍｏｎｄｓ两级命名体系。结果：广州ＨＣＶ基因型分布１ａ占７６％、１ｂ占４９５％、２ａ占２３８％、２ｂ占１９％、３ａ占２９％、１ｂ＋２ａ混合型占９５％、未定型占５％，同国内其它地区一些学者所报道的结果相似。结论：ＨＣＶ基因型分布不存在先前认为的地理上的南北差异。不同基因型传播途径没有差异，均以血液传播为主（８２９％）。１型及混合型感染病情较重，干扰素疗效较差 Objective: To investigate the distribution of hepatitis C virus (HCV) genotypes in Guangzhou and the relationship between HCV genotypes and clinical status. Methods: The cases were selected from Guangzhou residents. Genotyping was performed using Okamoto’s “type-specific primer PCR method”. The genotypes were named using internationally standardized Simmonds two-stage nomenclature. Results: The distribution of HCV genotype 1a accounted for 76%, 1b accounted for 495%, 2a accounted for 238%, 2b accounted for 19%, 3a accounted for 29%, 1b + 2a mixed accounted for 95% , Unsteady accounted for 5%, with some scholars in other parts of the country reported similar results. CONCLUSIONS: The genotype distribution of HCV does not have previously identified geographic differences between north and south. There was no difference in the transmission of different genotypes, both of which were mainly blood (82.9%). Type 1 and mixed infections are more serious and interferon is less effective