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目的：探讨ｍｄｍ－２基因表达和ｐ５３基因突变在星形细胞肿瘤发生发展中的作用及两者的相关性。方法：采用ＳＡＢＣ免疫组化法对１２０例星形细胞肿瘤进行检测。结果：在分化不良性星形细胞瘤及多形性胶质母细胞瘤中，ＭＤＭ２蛋白的阳性率分别为３０．００％和３５．２９％，突变型ｐ５３蛋白的阳性率分别为３５．００％和４４．１２％，均明显高于高分化星形细胞瘤（４．３５％和６．５２％）；突变型ｐ５３蛋白染色阴性的星形细胞肿瘤中，ＭＤＭ２蛋白阳性率（２６．１５％）明显高于其染色阳性的肿瘤（９．３８％）。结论：ＭＤＭ２蛋白及突变型ｐ５３蛋白在星形细胞肿瘤中的表达均与该肿瘤的分化程度有关；ｍｄｍ－２基因扩增及产物ＭＤＭ２蛋白过度表达，可能是突变型ｐ５３蛋白染色阴性的星形细胞肿瘤发生的分子基础
Objective: To investigate the role of mdm-2 gene expression and p53 gene mutation in the development of astrocytic tumors and their relationship. Methods: SABC immunohistochemical method was used to detect 120 cases of astrocytoma. Results: In poorly differentiated astrocytoma and glioblastoma, the positive rates of MDM2 protein were 30.00% and 35.29%, respectively. The positive rates of mutant p53 protein were 35.00 % And 44.12%, respectively, were significantly higher than those in well-differentiated astrocytoma (4.35% and 6.52%). The positive rate of MDM2 protein in astrocytic tumors with mutant p53 protein was 26.15 %) Was significantly higher than its staining-positive tumors (9.38%). Conclusion: The expression of MDM2 protein and mutant p53 protein in astrocytic tumors are all related to the degree of differentiation of the tumor. The mdm-2 gene amplification and product MDM2 protein overexpression may be the star-negative mutant of p53 protein Molecular basis of cell tumorigenesis