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Objective:To survey hemoplasmas infection in free ranging non-human primates from 8 provinces in Thailand. Methods:DNA from ethylenediaminetetraacetic acid blood of 262 free ranging non-human primates were identified as hemoplasmas using PCR and phylogenetic analysis based on16S rRNA anpB genes. Results:A total of 148 non-human primates (56.49％) were determined positive for Candidatus Mycoplasma haemomacaque, including 125Macaca fascicularis and 23Macaca mulatta. Hemoplasmas can cause anemia in monkey but all positive samples were healthy. The positive rates in male and female non-human primates were not significantly different. Conclusions:Candidatus Mycoplasma infection is prevalent in free rangingMacaca fascicularis andMacaca mulatta in Thailand.