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以四磺基铁酞菁(FeTSPc)作为过氧化物模拟酶催化H_2O_2与L-酪氨酸的荧光反应,建立了测定宽含量范围的H_2O_2及葡萄糖的方法.该法的线性范围为0.0～8.0×10~(-5)mol/LH_2O_2及0.0～2.O×10~(-4)mol/L葡萄糖,检测限分别为5.0×10~(-8)mol/L及6.0×10~(-8)mol/L将该反应与氧化酶催化反应偶联,测定人血清中葡萄糖的含量,取得了满意的结果.
The fluorescence reaction of H_2O_2 with L-tyrosine catalyzed by FeTSPc as a peroxide mimic enzyme was established and a method was developed for the determination of H_2O_2 and glucose in a wide range of content.The linear range of the method was 0.0 ~ 8.0 The detection limits were 5.0 × 10 ~ (-8) mol / L and 6.0 × 10 ~ (-) mol / L, respectively. 8) mol / L The reaction was coupled with oxidase catalytic reaction, the determination of glucose in human serum content and achieved satisfactory results.