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用抗Ⅳ型胶原单克隆抗体免疫组化法对实验性铬酸钠中毒大鼠肾进行了研究。结果显示：对照组肾组织各部基膜Ⅳ型胶原免疫组化染色呈弱阳性。一次气管内注入铬酸钠０．２ｍｇ／ｋｇ２天后，在肾组织出现损伤的同时，其染色亦增强呈阳性。一次气管内注入铬酸钠０．９８ｍｇ／ｋｇ后第２至２８天其染色均较对照组增强，以第４至２１天为甚。Ⅳ型胶原在肾组织各部基膜的染色强弱与肾组织损伤程度相关。结果提示Ⅳ型胶原可作为一个指标来评价铬等其它重金属所致肾损伤的发生、发展和转归。
Experimental renal sodium chromate poisoning was performed using anti-type IV collagen monoclonal antibody immunohistochemistry. The results showed that in the control group, the expression of type Ⅳ collagen in the basement membrane of the control group was weakly positive. An intra-tracheal injection of sodium chromate 0.2mg / kg 2 days after injury in the renal tissue at the same time, its staining also increased positive. After an intratracheal injection of sodium chromate 0.98mg / kg 2 to 28 days after staining were enhanced compared with the control group, to 4 to 21 days is even. Type Ⅳ collagen in the various parts of the renal tissue staining of the basement membrane and renal damage related to the degree. The results suggest that type Ⅳ collagen can be used as an index to evaluate the occurrence, development and prognosis of renal injury caused by other heavy metals such as chromium.