系统感染长叶车前花叶病毒的青菜叶片叶柄分别作冰冻切片,运用免疫酶标技术对叶片叶柄内的病毒进行显色定位。观察到病毒与酶标抗体结合而显色的现象广泛地存在于叶片的叶肉细胞内和叶柄的薄壁细胞内,在维管束周围的薄壁细胞和木质部与韧皮部之间的形成层细胞中尤甚。对叶片内的病毒作免疫荧光定位,荧光显色部位基本上与酶标显色部位一致。健叶片叶柄均不显色。 The leaf petioles of cabbage leaves infected with long-leaved mosaic virus were systematically infected by cryopreservation system. The colorimetric localization of the virus in the leaf petioles was carried out by using enzyme-linked immunosorbent assay (ELISA). It is observed that the phenomenon that the virus binds with the enzyme-labeled antibody is extensively present in the mesophyll cells of leaves and in the parenchyma cells of the petiole. In the parenchyma cells surrounding the vascular bundles and the cambium cells between xylem and phloem very. The virus within the leaves for immunofluorescence localization, fluorescent chromogenic sites basically consistent with the enzyme-labeled chromogenic sites. Health leaves petiole are not color.