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目的:评价荧光染色法在痰涂片抗酸杆菌镜检中的临床价值。方法:收集330例结核病门诊就诊病人的痰液,用荧光染色法与萋-尼氏抗酸染色法检测痰液中抗酸杆菌,比较两种方法的阳性检出率及阳性分级标准差异。结果:荧光染色法阳性检出率为21.3%,萋-尼氏抗酸染色法阳性检出率为21.6%,结果无统计学意义(P>0.05)。阳性标本量化级别在1-9条/50个视野(荧光染色)和1-8条/300个视野(萋-尼氏抗酸染色)时,其阳性样本百分比分别为2.2%和9.6%,差异有统计学意义(P<0.05);在其他分级标准下,两种方法阳性百分比差异不大(P>0.05)。结论:痰涂片荧光染色法更适合临床推广。
Objective: To evaluate the clinical value of fluorescent staining in sputum smear acid-fast bacilli microscopy. Methods: The sputum samples from 330 TB clinic patients were collected. The acid-fast bacilli in sputum were detected by fluorescence staining and 萋-Nisshore acid-fast staining. The positive detection rates and positive standard differences of the two methods were compared. Results: The positive rate of fluorescence staining was 21.3%, and the positive rate of 萋 -Ni-acid antacid staining was 21.6%. There was no significant difference (P> 0.05). The percentage of positive samples was 2.2% and 9.6% for the positive samples at 1-9 bands / 50 fields (fluorescence staining) and 1-8 bands / 300 fields (萋 -Nitz’s acid-fast staining) (P <0.05). The positive percentages of other two methods were not significantly different (P> 0.05). Conclusion: Sputum smear fluorescence staining is more suitable for clinical promotion.