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目的对2013-2015年杭州地区腹泻患者副溶血弧菌(Vibrio parahaemolyticus)毒力基因的携带和耐药进行调查研究。方法可疑菌株用全自动细菌鉴定仪(VITEK2Compact,生物梅里埃,法国)进行鉴定,用PCR方法检测其8种毒力基因,用纸片扩散法检测13种抗生素的敏感性。结果 120株副溶血弧菌中,tdh+或trh+的致病菌株占95.0%(114株),tdh-且trh-的非致病株占5.0%(6株)。所有120株副溶血弧菌均含有T3SS1基因,T3SS2α基因主要存在于tdh+/trh-的菌株中,T3SS2β存在于trh+的菌株中,2株tdh-的菌株首次检出T3SS2α基因。本研究中大流行菌株占64.2%(77株),非大流行菌株占35.8%(43株)。多达65.8%的副溶血弧菌对氨苄西林耐药,而90.0%以上的副溶血弧菌对其他抗生素包括喹诺酮类、第三代头孢菌素类、氨基糖苷类、四环素类和磺胺类抗菌药物较敏感。结论杭州地区腹泻来源的副溶血弧菌多为大流行菌株,对常见抗菌药物敏感性高,但耐药率有所上升,要加强监测并引起临床重视。
Objective To investigate the virulence and virulence of Vibrio parahaemolyticus in diarrhea patients in Hangzhou during 2013-2015. Methods Susceptible strains were identified by automatic bacterial analyzer (VITEK2Compact, Bio Merill, France). Eight virulence genes were detected by PCR and the sensitivity of 13 antibiotics was tested by disk diffusion method. Results Among 120 strains of Vibrio parahaemolyticus, 95.0% (114 strains) of tdh + or trh + were pathogenic strains, and 5.0% (6 strains) of tdh- and trh- non-pathogenic strains. All 120 Vibrio parahaemolyticus contained T3SS1 gene, T3SS2α gene mainly existed in tdh + / trh-, T3SS2β was in trh + strain, and two strains of tdh- were the first to detect T3SS2α gene. In this study, the prevalence of pandemic strains accounted for 64.2% (77 strains), non-pandemic strains accounted for 35.8% (43 strains). As many as 65.8% of V. parahaemolyticus were resistant to ampicillin, while 90.0% of V. parahaemolyticus were resistant to other antibiotics including quinolones, third generation cephalosporins, aminoglycosides, tetracyclines and sulfa antibiotics More sensitive. Conclusion Most of the strains of Vibrio parahaemolyticus originated from diarrhea in Hangzhou are mostly pandemic strains, which are sensitive to common antibiotics, but the drug resistance rate has risen. Monitoring and clinical importance should be strengthened.