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抗生素效价测定,1990版《中国药典》规定为生物测定法。在实际操作中,微生物测定法的正确性受很多因素影响,为了使测定的结果真实地反映客观情况,符合统计学原理,应将测定结果按生物统计进行可靠性检验、可信限计算,但数据处理比较繁琐,费工费时。为了克服繁琐的计算,根据《中国药典》规定的(2,2)法(1)(2),利用岛津 C-R4A色谱数据处理机编制了 BASIC 程序(1),该程序具有步骤清晰,操作简便等优点,只要按屏幕提示逐项输入已知的数据和测得值,即能报告统计结果、
Determination of antibiotic potency, 1990 edition of “Chinese Pharmacopoeia” provides for bioassay. In practice, the correctness of the microbial assay is affected by many factors. In order to make the determination result truly reflect the objective situation and accord with the statistical principle, the reliability of the determination result should be biometrically tested, and the confidence limit should be calculated. However, Data processing is more complicated, time-consuming work. In order to overcome the tedious calculation, BASIC program (1) was prepared by using Shimadzu C-R4A chromatographic data processor according to (2,2) method (1) (2) stipulated in “Chinese Pharmacopoeia” Easy to operate and so on, as long as you press the screen prompts to enter known data and measured values, which can report the statistical results,