论文部分内容阅读
目的:研究罗汉果抑制大肠埃希菌生物膜(BBF)有效部位的化学成分及单体活性。方法:以大肠埃希菌BBF为模型对罗汉果的有效部位进行筛选,采用柱色谱等方法分离其有效部位中的化学成分,鉴定其结构,并研究它们对大肠埃希菌生物膜的影响。结果:95%乙醇洗脱部位为有效部位,并从中分离得到3个化合物,分别为十六烷酸(1),环-(亮氨酸-异亮氨酸)(2),谷甾醇-3-O-葡萄糖(3)。在质量浓度为1 000 mg·L~(-1)时,化合物1,2,3对大肠埃希菌生物膜的抑制率分别为51.47%,76.34%,67.01%。结论:化合物3首次从罗汉果中分离得到。
Objective: To study the inhibition of the chemical composition and monomer activity of Lo Han Guo on the active site of Escherichia coli biofilm (BBF). Methods: Escherichia coli BBF was used as a model to screen the effective parts of Siraitia grosvenorii. The chemical components of the Siraitia grosvenorii were isolated by column chromatography and their structures were identified. Their effects on the biofilm formation of Escherichia coli were also studied. Results: The elution fraction of 95% ethanol was the effective site, and three compounds were isolated from them, which were hexadecanoic acid (1), cyclo- (leucine-isoleucine) (2) -O-glucose (3). The inhibitory rates of compounds 1, 2 and 3 on Escherichia coli biofilm were 51.47%, 76.34% and 67.01% at the concentration of 1 000 mg · L -1, respectively. Conclusion: Compound 3 was isolated from Luo Han Guo for the first time.