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目的 :分析非小细胞肺癌 (non smallcelllungcancer ,NSCLC)病人的T细胞受体 (Tcellreceptor ,TCR)Vβ亚家族T细胞的分布及其克隆性增殖特点。方法 :利用RT PCR和基因扫描方法分析 3例NSCLC外周血和骨髓单个核细胞中 2 4个TCRVβ基因的CDR3长度 ,了解TCRVβT细胞的分布及其克隆性。 结果 :3例病人仅存在 1～ 5个TCRVβ亚家族 ,主要为Vβ3、Vβ7和Vβ9,2例病人出现Vβ7寡克隆性增殖T细胞 ,另 1例发现Vβ3的寡克隆T细胞。结论 :NSCLC病人外周血或骨髓的TCRVβ亚家族出现倾斜性分布和克隆性增殖T细胞 ,主要为Vβ3和Vβ7亚家族T细胞 ,这可能是由肺癌细胞相关抗原引起的特异性免疫反应的T细胞克隆
Objective: To analyze the distribution and clonal proliferation characteristics of T-cell receptor (TCR) Vβ subfamily T cells in patients with non-small cell lung cancer (NSCLC). Methods : RT PCR and gene scanning methods were used to analyze the CDR3 length of 24 TCR Vβ genes in peripheral blood and bone marrow mononuclear cells of 3 patients with NSCLC to know the distribution and clonality of TCR Vβ T cells. Results: There were only 1 to 5 TCRVβ subfamily in 3 patients, mainly Vβ3, Vβ7 and Vβ9. Vβ7 oligoclonal clonal T cells were found in 2 patients and oligoclonal T cells in Vβ3 were found in another case. Conclusion: The TCR Vβ subfamily in peripheral blood or bone marrow of patients with NSCLC presents a skewed distribution and clonally proliferating T cells, mainly Vβ3 and Vβ7 subfamily T cells, which may be specific immune response T cells caused by lung cancer cell-associated antigens. clone