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[目的]探讨锯叶棕提取物对多烯紫杉醇抗MM细胞增殖的影响。[方法]将体外培养的U266和PRMI8226细胞与0-2μL/mL的不同浓度锯叶棕提取物共孵育,应用MTT比色法测定细胞增殖活性;同时应用台盼蓝不相容实验检测锯叶棕提取物与多烯紫杉醇单独使用或合并使用时的细胞成活率。[结果]锯叶棕提取物对U266细胞与RP-M18226细胞的增殖具有明显的抑制作用,抑制生长50%(ED_(50S))的有效剂量分别为1.2和0.7μL/mL;U266细胞用锯叶棕提取物或多烯紫杉醇预处理,其细胞成活率分别为79.7%或90.1%,两者联合应用细胞成活率为51.6%(P<0.001);RPMI8226细胞用锯叶棕提取物或多烯紫杉醇预处理,其细胞成活率分别为73.7%或80.1%,两者联合应用细胞成活率为55.6%(P<0.001)。[结论]锯叶棕提取物抑制MM细胞增殖并增强多烯紫杉醇抗MM细胞增殖作用。
[Objective] To investigate the effect of Saw Palmetto extract on proliferation of docetaxel-resistant MM cells. [Method] U266 and PRMI8226 cells cultured in vitro were incubated with 0-2μL / mL different concentrations of Saw Palmetto extract, MTT colorimetric assay was used to measure the cell proliferation activity. At the same time, trypan blue exclusion assay Cell viability of palm extract and docetaxel alone or in combination. [Result] Saw palmetten brown extract had obvious inhibitory effect on the proliferation of U266 cells and RP-M18226 cells, and the effective dosages of inhibiting the growth of 50% (ED 50S) were 1.2 and 0.7 μL / mL, respectively. U266 cells were seeded with saw The cell viability of leaf palm extract or docetaxel pretreatment was 79.7% or 90.1%, respectively. The survival rate of the combination of the two was 51.6% (P <0.001). The RPMI8226 cells were treated with Saw Palmetto extract or polyene Paclitaxel pretreatment, the cell survival rates were 73.7% or 80.1%, the combination of the two cell survival rate was 55.6% (P <0.001). [Conclusion] The Saw Palmetto extract inhibited the proliferation of MM cells and enhanced the proliferation of docetaxel against MM cells.