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将用合成的匙孔形虫戚血蓝蛋白-甲醛-河豚毒素(KLH-HCHO-TTX)连接物免疫的BALB/c小鼠的脾细胞与小鼠骨髓瘤细胞系Sp2/0融合,经3~4次亚克隆,建立了3个稳定分泌抗河豚毒素的杂交瘤细胞株,分别命名为1G3、4G3、6D9。其中1G3和4G3分属IgG2b亚类,6D9属IgG1亚类。腹水的抗体稀释度为1∶105~1:5×106。用竞争抑制性酶联免疫吸附试验(ELISA)测定三种抗体的最低反应浓度为1×10-3mg/L。
Spleen cells of BALB / c mice immunized with the synthetic keyhole limpet hemocyanin-formaldehyde-tetrodotoxin (KLH-HCHO-TTX) conjugate were fused with the mouse myeloma cell line Sp2 / 0 via 3 ~ 4 subclones, established three stable secreted anti-tetrodotoxin hybridoma cell lines, respectively, named 1G3, 4G3, 6D9. Among them, 1G3 and 4G3 belong to IgG2b subclass and 6D9 belong to IgG1 subclass. Ascites antibody dilution 1:105 ~ 1: 5 × 106. The minimum reaction concentration of the three antibodies was 1 × 10-3 mg / L as determined by competitive inhibition enzyme-linked immunosorbent assay (ELISA).