论文部分内容阅读
目的 探究七氟醚对结直肠癌细胞中细胞外基质金属蛋白酶诱导因子 (CD147) 及基质金属蛋白酶-2 (MMP-2) 表达的影响及其生物学作用.方法 采用0％、1.7％、3.4％及5.1％气体浓度七氟醚处理结直肠癌细胞HCT116 6 h, 分别设置为对照组、低剂量组、中剂量组及高剂量组, 采用CCK-8试剂检测不同组细胞0 h、24 h、48 h、72 h及96 h细胞活力, 采用Transwell实验检测不同组细胞侵袭及迁移能力, 采用实时荧光定量PCR (q PCR) 技术检测不同组细胞CD147及MMP-2 m RNA表达水平, Western blot检测CD147及MMP-2蛋白的表达水平.结果 CCK-8实验结果显示:中剂量组及高剂量组细胞活力在培养至72 h及96 h时显著低于对照组, 低剂量组细胞活力在培养至96 h时显著低于对照组, 差异具有统计学意义 (P＜0.05) .Transwell实验结果显示:细胞侵袭模型中, 中剂量组及高剂量组侵袭细胞数显著低于对照组细胞;细胞迁移模型中, 中剂量组及高剂量组迁移细胞数显著低于对照组细胞, 差异具有统计学意义 (P＜0.05) .q PCR结果显示:中剂量组及高剂量组CD147 m RNA表达水平显著低于对照组, 高剂量组MMP-2 m RNA表达水平显著低于对照组.Western blot实验结果显示:对照组、低剂量组、中剂量组及高剂量组CD147及MMP-2蛋白表达水平均呈梯度下调.结论 七氟醚可下调CD147及MMP-2的表达, 进而抑制结直肠癌细胞HCT116增殖、侵袭及迁移能力.“,”Objective To investigate the effects of sevoflurane on the expression of extracellular matrix metalloproteinase inducer (CD147) and matrix metalloproteinase-2 (MMP-2) in colorectal cancer cells and its biological functions.Methods 0％, 1.7％, 3.4％ and 5.1％sevoflurane were used to treat colorectal cancer cells HCT116 for 6 h, which were set as control group, low dose group, medium dose group and high dose group respectively.CCK-8 reagent was used to detect the cell viability of different groups in 0 h, 24 h, 48 h, 72 h and 96 h.Transwell test was used to detect the cell invasion and migration of different groups.Quantitative Real-time PCR (qPCR) technology was used to detect the expression level of CD147 and MMP-2 m RNA in different groups.Western blot was used to detect the expression level of CD147 and MMP-2 protein.Results The results of CCK-8 experiment showed that the cell viability of medium dose group and high dose group was significantly lower than that of control group at72 h and 96 h.The cell viability of low dose group was significantly lower than that of control group at 96 h, and the difference was statistically significant (P＜0.05).The results of Transwell experiment showed that in the cell invasion model, the number of invasive cells in the high dose group was significantly lower than that of the control group, and the number of migrate cells in the medium dose group and the high dose group was significantly lower than that of the control group, and the difference was statistically significant (P＜0.05).qPCR results showed that the expression level of CD147 m RNA in the medium dose group and high dose group was significantly lower than that in the control group, and the expression level of MMP-2 m RNA in high dose group was significantly lower than that in the control group.The results of Western blot showed that the expression levels of CD147 and MMP-2 in the control group, lowdose group, middle dose group and high dose group were graded down.Conclusion Sevoflurane can down-regulate the expression of CD147 and MMP-2, and inhibit the proliferation, invasion and migration of HCT116 cells.