论文部分内容阅读
背景与目的:实验证明S100A4在多种恶性肿瘤细胞中高表达,它可能在恶性肿瘤细胞的侵袭和转移等过程中发挥重要作用。本研究探讨S100A4在人非小细胞肺癌中的表达及其与侵袭和转移的关系。方法:采用免疫组化SP法检测41例非小细胞肺癌及6例正常肺组织中S100A4蛋白的表达水平。结果:S100A4在非小细胞肺癌中的阳性率(70.7%)显著高于正常肺组织(16.7%)(P<0.05);在肺腺癌中的阳性率(90.0%)明显高于肺鳞癌(52.4%)(P<0.01)。在TNM分期中,S100A4在Ⅲ+Ⅳ期、Ⅱ期和Ⅰ期的阳性率分别为100.0%、66.7%和30.0%,Ⅲ+Ⅳ期明显高于Ⅱ期和Ⅰ期(P<0.01),但是Ⅱ期和Ⅰ期间无显著性差异(P>0.05)。S100A4的阳性率在有淋巴结转移的非小细胞肺癌中(90.0%)明显高于无淋巴结转移组(52.4%)(P<0.01),且S100A4的表达与淋巴结转移密切相关(r=0.480,P=0.001)。肿瘤体积增大(<3cm,≥3cm),S100A4的阳性率明显升高(44.4%,91.3%)(P<0.001),且相关性显著(r=0.288,P=0.017)。S100A4的阳性率与非小细胞肺癌的病理分级无明显相关性(P>0.05)。结论:S100A4在非小细胞肺癌中的表达上调,且与淋巴结转移、TNM分期和肿瘤大小密切相关,提示S100A4与非小细胞肺癌的侵袭和转移有关。
BACKGROUND & OBJECTIVE: Experiments have shown that S100A4 is highly expressed in a variety of malignant tumor cells. It may play an important role in the invasion and metastasis of malignant tumor cells. This study was to investigate the expression of S100A4 in human non-small cell lung cancer and its relationship with invasion and metastasis. Methods: Immunohistochemical SP method was used to detect the expression of S100A4 protein in 41 cases of non-small cell lung cancer and 6 cases of normal lung tissues. Results: The positive rate of S100A4 in non-small cell lung cancer (70.7%) was significantly higher than that in normal lung tissue (16.7%) (P<0.05); the positive rate in lung adenocarcinoma (90.0%) was significantly higher than that in lung squamous cell carcinoma. (52.4%) (P<0.01). In the TNM staging, the positive rates of S100A4 in stage III+IV, stage II, and stage I were 100.0%, 66.7%, and 30.0%, respectively. Stage III+IV was significantly higher than stage II and stage I (P<0.01), but There was no significant difference between phase II and phase I (P>0.05). The positive rate of S100A4 was significantly higher in non-small cell lung cancer with lymph node metastasis (90.0%) than in the group without lymph node metastasis (52.4%) (P<0.01), and the expression of S100A4 was closely related to lymph node metastasis (r=0.480, P). =0.001). The tumor volume increased (<3cm, ≥3cm), and the positive rate of S100A4 increased significantly (44.4%, 91.3%) (P<0.001), and the correlation was significant (r=0.288, P=0.017). The positive rate of S100A4 was not significantly associated with the pathological grade of NSCLC (P>0.05). Conclusion: The expression of S100A4 is up-regulated in non-small cell lung cancer, and it is closely related to lymph node metastasis, TNM stage and tumor size, suggesting that S100A4 is related to the invasion and metastasis of non-small cell lung cancer.