严格的对照是免疫组化染色的重要环节,采用膜膜相对染色法可以使实验切片和对照切片,两者的条件完全一致,而且也节省抗体等试剂。 1 操作步骤 将实验切片与对照切片或染相同抗体的两张切片相对,事先在二张切片中任何一张的两端贴上三层不干胶条,备两者合拢时组织膜间留出薄层间隙,将实验切片与对照切 Strict control is an important part of immunohistochemical staining, the use of membrane staining method can make the experimental sections and control sections, both the conditions are exactly the same, but also save antibodies and other reagents. 1 steps the experimental section and the control section or the same antibody staining two sections relative to the two sections in advance of any one of the two sections affixed to the three layers of self-adhesive strips, preparation of the two organizations together when the film left behind Lamellar gap, the experimental sections cut with the control