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本文应用抗增殖细胞核抗原的单克隆抗体ＰＣ１０，用ＬＳＡＢ免疫组化方法对４０例胃癌标本进行研究。结果发现：胃癌不同分化程度各组中ＰＣＮＡ指数存在差异（Ｐ＜０．０２），低分化腺癌＞中分化腺癌＞高分化腺癌；淋巴结癌转移阳性组ＰＣＮＡ指数显著高于无淋巴结转移组（Ｐ＜０．０５）；癌细胞浸润达肌层、浆膜层者ＰＣＮＡ指数显著高于粘膜内癌（Ｐ＜０．０１）。结果显示：此方法是在石蜡切片上检测胃癌增殖指数的一个有效手段，并对判断胃癌患者的预后有一定作用。
In this study, monoclonal antibody PC10 against PCNA was used to study 40 gastric cancer specimens by LSAB immunohistochemistry. The results showed that there were differences in PCNA index between different groups of gastric cancer (P<0.02), poorly differentiated adenocarcinoma>medium-differentiated adenocarcinoma>highly differentiated adenocarcinoma; the PCNA index of lymph node metastasis positive group was significantly higher than that of no lymph node metastasis. In the group (P<0.05), the PCNA index of cancer cells infiltrating into muscular layer and serosal layer was significantly higher than that in intramucosal cancer (P<0.01). The results showed that this method is an effective method to detect the proliferation index of gastric cancer on paraffin sections, and it has a certain effect on the prognosis of gastric cancer patients.