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用限制性内切酶TaqI和C4基因5 ̄'末端探针pAT-A检测58份湖北地区健康献血员基因组DNA的C4基因限制性片段长度多态性(RFLP),观察到7.0kb、6.0kb和5.4kb三种C4基因片段,它们共组成三种常见的C4基因RFLP表型,即A:7.05-4kb(频率为24.14%));B:7.0-6.0kb(频率为20.69%)和C:7.0-6.0-5.4kb(频率为55.17%,在所检测的样本中未观察到6.4kb的C4基因片段。
The C4 gene restriction fragment length polymorphism (RFLP) of 58 healthy volunteers in Hubei province was detected by restriction endonuclease TaqI and 5 ’end probe pAT-A of C4 gene. .0kb and 5.4kb three C4 gene fragments, which together make up the three common RFLP phenotypes C4 gene, namely A: 7.05-4kb (frequency of 24.14%)); B: 7.0-6 .0kb (frequency 20.69%) and C: 7.0-6.0-5.4kb (frequency 55.17%). No 6.4kb C4 gene fragment was observed in the samples tested.