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用微悬浮聚合技术制备微米级顺磁性的聚合物微球,并通过水解反应在其表面生成丰富的羟基,进而通过二乙烯基砜活化,在其表面修饰2-巯基嘧啶,实现磁性微球对人体免疫球蛋白G(IgG)的特异性识别.进一步探讨了不同解吸附环境,如pH值、温度、离子种类、离子强度等条件,对这种特异性吸附行为的影响.采用此磁性分离技术后,分离抗体的纯度超过92%,抗体活性高于99%.通过连续分离工艺,IgG的分离效率达到86.8%.
Microspheres with microspheres were prepared by micro-suspension polymerization, and the hydroxyl groups were formed on the surface of the polymer microspheres by hydrolysis. Then, activated by divinyl sulfone, 2-mercaptopyrimidines were modified on the surface to achieve magnetic microspheres The specific recognition of human immunoglobulin G (IgG) was further explored.The effects of different desorption environments such as pH value, temperature, ionic species and ionic strength on this specific adsorption behavior were also investigated.Using this magnetic separation technique After purification, the purity of the isolated antibody was over 92% and the activity of the antibody was above 99%. The separation efficiency of IgG reached 86.8% through the continuous separation process.