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目的通过研究脂蛋白糖基化后易被氧化修饰的性质来探讨胰岛素抵抗患者易于发生动脉粥样硬化的机理。方法用葡萄糖和低密度脂蛋白(LDL)共同温育后得到糖基化脂蛋白(Glu-LDL),并用琼脂糖电泳法和硫代巴比妥酸(TBA)比色法鉴定糖基化的程度。用Cu2+和小鼠腹腔巨噬细胞分别和Glu-LDL共同温育,检测TBA反应物(TBARS)含量及电泳迁移速度。结果Glu-LDL和未糖基化LDL分别与Cu2+共同温育8h后,二者TBARS含量具有显著性差异(P<0.001);Glu-LDL和LDL分别与小鼠腹腔巨噬细胞共同温育24及48h后,二者TBARS含量亦具有显著性差异(P<0.05),电泳迁移率也有相应改变。结论脂蛋白糖基化后易被氧化修饰,说明其稳定性下降。
Aim To investigate the mechanism of atherosclerosis in insulin resistance patients by studying the properties of lipoproteins susceptible to oxidative modification after glycosylation. Methods Glycosylated lipoproteins (Glu-LDL) were obtained by incubation with glucose and low density lipoprotein (LDL), and glycosylated lipoproteins were identified by agarose electrophoresis and thiobarbituric acid (TBA) degree. Cu2 + and mouse peritoneal macrophages were incubated with Glu-LDL respectively to detect the content of TBARS and the electrophoretic mobility. Results After incubation with Cu2 + for 8 hours, Glu-LDL and unglycosylated LDL had significant difference (P <0.001), and Glu-LDL and LDL were respectively co-incubated with mouse peritoneal macrophages After incubation for 24 and 48 hours, the TBARS content in both groups also had significant difference (P <0.05), and the electrophoretic mobility also changed accordingly. Conclusion Lipoprotein glycosylation easily oxidative modification, indicating that the stability decreased.