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在整体水平观察卵巢切除 ,雌激素替代对小鼠学习和记忆行为的影响及脑内单胺递质含量的变化 ,同时进一步从离体水平观察了雌激素对过氧化氢 (H2 O2 )致原代培养海马神经细胞的保护作用 .通过避暗实验 (被动回避反应 )观察了雌激素对学习记忆行为的影响 ,高效液相色谱法电化学检测器检测了脑内单胺递质含量的变化 ,MTT法观察了雌激素对原代培养海马神经细胞存活率的影响 .在避暗实验中 ,卵巢切除小鼠记忆保持率明显下降 ,脑内多巴胺及 5 羟色胺含量亦明显降低 .雌激素替代对上述指标有明显的改善作用 .雌激素提前 2h给予可显著拮抗H2 O2 所致胚胎大鼠原代培养海马神经细胞存活率的降低 .雌激素缺乏可影响学习记忆功能 ,且与脑内单胺递质含量变化具有密切关系 .进一步研究表明 ,雌激素具有直接的神经保护作用 ,保护神经元免受氧化应激的损伤 .
To observe the effects of ovariectomy and estrogen replacement on the learning and memory behaviors of mice and the contents of monoamine neurotransmitters in the brain of rats at the whole level. At the same time, the effect of estrogen on H2O2 The effect of estrogen on learning and memory behavior was observed by dark-avoidance experiment (passive avoidance reaction). The content of monoamine neurotransmitters in brain was detected by high performance liquid chromatography electrochemical detector, The effect of estrogen on the survival rate of primary cultured hippocampal neurons was observed by MTT assay.During the dark-avoidance experiment, the memory retention rate of ovariectomized mice was significantly decreased and the contents of dopamine and serotonin in the brain were also significantly decreased.Examination of estrogen on the 2 h before administration of estrogen could significantly reduce the survival rate of hippocampal neurons in primary cultured embryonic rat induced by H2O2.The lack of estrogen can affect the learning and memory function and the effect of monoamine neurotransmitters Content change is closely related to further studies have shown that estrogen has a direct neuroprotective effect to protect neurons from oxidative stress damage.